P.V. you are way ahead of the game for using an actual product, which has been argued on this forum many times from profiling companies to oven manufacturers as the only true representation of a profile.
To answer your question, a lot will depend on the board material, the temperature, and the amount of time that the board spends above the "glass transition temperature."
Above a certain temperature the FR4 material outgases and actually loses weight. In our tests at KIC, once the board loses about 3-5% of it's original weight, it is no longer a close enough representation of the original board to create a valid profile.
In other words, your golden board is not so golden anymore. Our tests showed somewhere between 5 and 10 runs before your PCB is compromised. You also need to watch the thermocouple attachment closely.
We found when attaching the TCs with aluminum tape, we would have to re-attach the TCs at least every five runs. Again a lot depends on the profile temperature.
Lead Free profiles are typically hotter and will wear out the TC attachment quicker. You might be tempted to use high temperature solder or epoxy, but a recent study concluded by RIT to be released soon shows this to be a very poor choice.
Another alternative often proposed as a solution is to use a solid state fixture, but a large bulky thermal mass is no closer to a true representation of your product than your PCB that has been profiled repeatedly. I wrote a somewhat provocative blog on this topic, where I compare some fixtures to sending a brick through your oven.
There are better alternatives to all of this. My customers will use KIC's Auto-Focus to create their first profile. What is nice about this tool, it does not require you to run a physical profile on your first go around, since a solution is given to you based off of a database of prior work.
In practice, many customers will use this solution as their set-up and run production or they decide to thermocouple up their board anyway and run the profile but optimize using another tool of ours called Navigator that drives the profile deep within their process specs.
Either way, you never profile a "sellable" production board more than twice. You have dead on accurate profiles because you are basing your work on true production boards, no bricks no scrap no guesses.
Hope this helps.